Analytical Data
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Gene name
NEFH
- Application
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Alternative Names
NEFH；KIAA0845；NFH；Neurofilament heavy polypeptide
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P12036
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Expression Region
1-413aa
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AA Sequence
MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRI
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Molecular Weight
65.6kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Nefh (Neurofilament heavy chain) is a key structural protein found in the axonal cytoskeleton of neurons and is critical for maintaining neuronal integrity and function. Research on NEFH recombinant proteins has gained attention due to their potential implications in neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS), where neurofilament accumulation and axonal damage are prominent features. By studying NEFH in various contexts, scientists aim to understand its role in neurodegeneration and repair processes. Additionally, recombinant NEFH proteins can serve as valuable tools for investigating cellular mechanisms, identifying biomarkers, and developing therapeutic strategies. Advances in recombinant DNA technology have enabled the production of high-purity NEFH proteins, facilitating detailed studies of their biochemical properties and interactions with other cytoskeletal components. Moreover, these proteins may play a role in the development of assays for neurodegenerative disease diagnostics and in testing potential drugs that target neurofilament dynamics. Overall, the exploration of NEFH recombinant proteins not only enhances our understanding of neuronal resilience and pathology but also opens new avenues for the development of innovative treatments for neurodegenerative disorders.
