Analytical Data
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Gene name
NEK2
- Application
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Alternative Names
NEK2；NEK2A；NLK1；Serine/threonine-Protein kinase Nek2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51955
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Expression Region
1-445aa
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AA Sequence
MPSRAEDYEV LYTIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEAEKQ MLVSEVNLLR ELKHPNIVRY YDRIIDRTNT TLYIVMEYCE GGDLASVITK GTKERQYLDE EFVLRVMTQL TLALKECHRR SDGGHTVLHR DLKPANVFLD GKQNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQMNR MSYNEKSDIW SLGCLLYELC ALMPPFTAFS QKELAGKIRE GKFRRIPYRY SDELNEIITR MLNLKDYHRP SVEEILENPL IADLVADEQR RNLERRGRQL GEPEKSQDSS PVLSELKLKE IQLQERERAL KAREERLEQK EQELCVRERL AEDKLARAEN LLKNYSLLKE RKFLSLASNP ELLNLPSSVI KKKVHFSGES KENIMRSENS ESQLTSKSKC KDLKKRLHAA QLRAQALSDI EKNYQLKSRQ ILGMR
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NEK2 (NIMA-related kinase 2) is a serine/threonine kinase that plays a crucial role in cell cycle regulation, particularly during mitosis. It is part of the NIMA family of kinases, which are evolutionarily conserved and essential for proper cell division. NEK2 is known to be involved in centrosome function, spindle assembly, and chromosome segregation, making it significant for maintaining genomic stability. Aberrant expression or dysregulation of NEK2 has been implicated in various cancers, suggesting that it may serve as a potential biomarker for tumor diagnosis and treatment. Due to its central role in cellular processes, NEK2 has garnered attention for the development of targeted therapies. Research into NEK2 recombinant proteins aims to elucidate its functional mechanisms and interactions within the cell cycle. By producing recombinant NEK2, scientists can investigate its kinase activity, identify its substrates, and understand how its dysregulation contributes to tumorigenesis. This knowledge not only enhances our understanding of cell biology but also paves the way for novel therapeutic strategies that could inhibit NEK2 in cancer treatment, offering hope for more effective interventions against malignancies associated with its overexpression. Thus, the study of NEK2 recombinant proteins represents a vital frontier in both basic biology and clinical research, highlighting the importance of kinases in health and disease.
