Analytical Data
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Gene name
EPAS1
- Application
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Alternative Names
Basic helix loop helix PAS protein MOP2; Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; ECYT4; Endothelial PAS domain containing protein 1; Endothelial pas domain protein 1; Endothelial PAS domain-containing protein 1; EPAS 1; EPAS-1; EPAS1; EPAS1_HUMAN; HIF 1 alpha like factor; HIF 2 alpha; HIF-1-alpha-like factor; HIF-2-alpha; HIF2-alpha; HIF2A; HLF
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99814
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Expression Region
28-347aa
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AA Sequence
SKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEI
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Molecular Weight
60.83 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EPAS1, also known as HIF-2α (Hypoxia-Inducible Factor 2 Alpha), plays a pivotal role in cellular response to hypoxia, which is a condition of reduced oxygen availability. It is a member of the hypoxia-inducible factor family, which regulates various physiological processes, including erythropoiesis, angiogenesis, and metabolism in response to low oxygen levels. Dysregulation of EPAS1 has been implicated in several pathological conditions, including cancer, where it promotes tumor growth and metastasis, and various cardiovascular diseases. Furthermore, mutations in the EPAS1 gene have been associated with congenital erythrocytosis, a disorder that leads to excessive red blood cell production. Consequently, the development of EPAS1 recombinant proteins has become an essential area of research. These proteins are useful for studying the mechanisms of hypoxia signaling and can serve as potential therapeutic targets or biomarkers for conditions related to altered oxygen levels. The recombinant form of EPAS1 can facilitate high-throughput screening of compounds that might modulate its activity, thus providing insight into novel treatment strategies for diseases influenced by hypoxia and EPAS1 dysfunction. Understanding the function and regulation of EPAS1, along with its interactions within cellular pathways, holds significant promise for advancing therapeutic approaches in various clinical settings.
