Analytical Data
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基因名
GJB5
- Application
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别名
GJB5; Gap junction beta-5 protein; Connexin-31.1; Cx31.1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95377
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表达区间
1-273aa
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氨基酸序列
MNWSIFEGLLSGVNKYSTAFGRIWLSLVFIFRVLVYLVTAERVWSDDHKDFDCNTRQPGCSNVCFDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREVQEKRHREAHGENSGRLYLNPGKKRGGLWWTYVCSLVFKASVDIAFLYVFHSFYPKYILPPVVKCHADPCPNIVDCFISKPSEKNIFTLFMVATAAICILLNLVELIYLVSKRCHECLAARKAQAMCTGHHPHGTTSSCKQDDLLSGDLIFLGSDSHPPLLPDRPRDHVKKTIL
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分子量
55.77 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GJB5, also known as Gap Junction Protein Beta 5, is a member of the connexin family, which plays a crucial role in cellular communication by forming gap junctions that facilitate the direct transfer of ions and small molecules between neighboring cells. Mutations in the GJB5 gene have been implicated in various human diseases, particularly skin disorders such as ectodermal dysplasias and hearing loss. The study of GJB5 recombinant protein is significant as it helps in understanding the structure-function relationships of gap junctions and their roles in maintaining cellular homeostasis. By producing and characterizing GJB5 recombinant protein, researchers aim to elucidate the molecular mechanisms underlying its function and the pathophysiology of diseases associated with its mutation. Furthermore, studying this protein can lead to insights into potential therapeutic strategies for conditions arising from impaired cell-to-cell communication. Given the essential nature of gap junctions in tissue integrity and intercellular signaling, the exploration of GJB5 and its interactions is vital for future biomedical applications, including drug development and gene therapy targeting connexin-related pathologies.
