Analytical Data
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Gene name
GPR114
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简介
GPR114, an adhesion G protein-coupled receptor (GPCR), crucially activates the adenylate cyclase pathway by coupling with G(s) subunit alpha. Isoform 1, displaying constitutive activity, elevates basal cyclic AMP (cAMP) levels and responds to mechanical stimulation, emphasizing GPR114's functional significance in intracellular signaling cascades and its role in cellular responses to mechanical cues. GPR114 Protein, Human (HEK293, Fc) is the recombinant human-derived GPR114 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Adhesion G-protein coupled receptor G5; G-protein coupled receptor 114; ADGRG5; PGR27
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IZF4
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Expression Region
E22-G184
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Protein Length
Partial
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Molecular Weight
57-75 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPR114, also known as the G protein-coupled receptor 114, plays a significant role in various biological processes and has garnered attention in the field of biomedical research. This receptor is a member of the adhesion G protein-coupled receptor (aGPCR) family, which is known for its involvement in cell signaling, immune response, and cell adhesion. Recent studies have suggested that GPR114 may be implicated in various pathological conditions, including cancer, inflammation, and neurological disorders, highlighting its potential as a therapeutic target. The investigation of recombinant GPR114 proteins facilitates a deeper understanding of its structure-function relationship, signaling pathways, and biological roles. Such studies may enable researchers to delineate the mechanisms by which GPR114 influences cellular processes and disease states. Additionally, the production and characterization of recombinant GPR114 proteins are essential for the development of specific inhibitors or activators, which could be invaluable in developing novel therapeutic strategies. Overall, the study of GPR114 and its recombinant proteins is a promising area of inquiry that may contribute to advances in drug discovery and the treatment of diseases associated with this receptor.
