Analytical Data
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Gene name
FZD3
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简介
FZD3 is a Wnt receptor that primarily activates the β-catenin canonical pathway, involving disheveled protein, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene induction. FZD3 Protein, Human (Cell-Free, His) is the recombinant human-derived FZD3 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Application
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Alternative Names
Frizzled-3; Fz-3; hFz3
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Species
Human
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Source
E. coli Cell-free
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPG1
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Expression Region
H23-A666
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AA Sequence
HSLFSCEPITLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHPMVNLDCSRDFRPFLCALYAPICMEYGRVTLPCRRLCQRAYSECSKLMEMFGVPWPEDMECSRFPDCDEPYPRLVDLNLAGEPTEGAPVAVQRDYGFWCPRELKIDPDLGYSFLHVRDCSPPCPNMYFRREELSFARYFIGLISIICLSATLFTFLTFLIDVTRFRYPERPIIFYAVCYMMVSLIFFIGFLLEDRVACNASIPAQYKASTVTQGSHNKACTMLFMILYFFTMAGSVWWVILTITWFLAAVPKWGSEAIEKKALLFHASAWGIPGTLTIILLAMNKIEGDNISGVCFVGLYDVDALRYFVLAPLCLYVVVGVSLLLAGIISLNRVRIEIPLEKENQDKLVKFMIRIGVFSILYLVPLLVVIGCYFYEQAYRGIWETTWIQERCREYHIPCPYQVTQMSRPDLILFLMKYLMALIVGIPSVFWVGSKKTCFEWASFFHGRRKKEIVNESRQVLQEPDFAQSLLRDPNTPIIRKSRGTSTQGTSTHASSTQLAMVDDQRSKAGSIHSKVSSYHGSLHRSRDGRYTPCSYRGMEERLPHGSMSRLTDHSRHSSSHRLNEQSRHSSIRDLSNNPMTHITHGTSMNRVIEEDGTSA
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Protein Length
Full Length of Mature Protein
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Molecular Weight
75.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FZD3, or Frizzled-3, is a key member of the Frizzled family of G protein-coupled receptors that plays a crucial role in the Wnt signaling pathway, which is essential for various biological processes, including embryogenesis, cell differentiation, and tissue homeostasis. Dysregulation of Wnt signaling, often mediated by Frizzled receptors, has been implicated in numerous diseases, including cancer and neurodegenerative disorders. The study of FZD3 recombinant protein has gained traction in recent years, as understanding its structure and function can provide insights into its role in cellular signaling and disease mechanisms. Research involving the production and characterization of FZD3 recombinant protein allows scientists to explore its interactions with Wnt ligands and other signaling molecules, assess its functional properties, and develop potential therapeutic strategies to modulate its activity. Furthermore, the use of recombinant FZD3 in experimental models can facilitate the design of novel compounds targeting Frizzled receptors, paving the way for innovative treatments in therapeutic areas where Wnt signaling is altered. Understanding the molecular basis and biological implications of FZD3's role in signaling pathways can illuminate its potential as a biomarker or therapeutic target in various clinical settings.
