Analytical Data
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Gene name
Frizzled-5
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简介
Frizzled-5 is a Wnt receptor that activates canonical Wnt/β-catenin signaling through WNT2, WNT10B, and WNT5A. It participates in a potential second pathway involving PKC and calcium flux, affecting Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5 Protein, Mouse (CHO, Fc) is the recombinant mouse-derived Frizzled-5 protein, expressed by CHO , with C-hFc labeled tag.
- Application
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Alternative Names
Frizzled-5; Fz-5; mFz5; Fzd5
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Species
Mouse
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Source
CHO
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9EQD0
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Expression Region
A27-P167
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Protein Length
Partial
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Molecular Weight
52-56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-5 (Fzd5) is a key member of the Frizzled family of G protein-coupled receptors, which are critical in the Wnt signaling pathway—a crucial pathway for cell proliferation, differentiation, and tissue homeostasis. The study of Fzd5 is particularly relevant in the context of embryonic development and various diseases, including cancer and neurodegenerative disorders, where aberrant Wnt signaling plays a pivotal role. Research has indicated that Fzd5 participates in the regulation of vascular development and angiogenesis, making it a potential target for therapeutic interventions in cardiovascular diseases. Furthermore, Fzd5's role in the central nervous system suggests its involvement in neuronal development and synaptic plasticity, presenting implications for neurodevelopmental conditions. Understanding the structural and functional properties of Fzd5, including its interaction with Wnt ligands and downstream signaling partners, is essential for the development of targeted therapies aimed at modulating the Wnt pathway. Advances in recombinant protein technologies have facilitated the production and analysis of Fzd5, enabling researchers to explore its mechanistic functions and potential as a biomarker or therapeutic target in various pathological contexts. As the body of literature surrounding Fzd5 expands, it holds promise for elucidating the complexities of Wnt signaling and its implications in health and disease.
