Analytical Data
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Gene name
NR1I2
- Application
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Alternative Names
Orphan nuclear receptor PAR1 Orphan nuclear receptor PXR Pregnane X receptor Steroid and xenobiotic receptor
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75469
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Expression Region
1-434aa
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Molecular Weight
69.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NR1I2, also known as Pregnane X Receptor (PXR), is a nuclear receptor that plays a crucial role in the regulation of genes involved in drug metabolism and excretion. Research into NR1I2 has gained significant attention due to its involvement in the body's response to xenobiotics, including pharmaceuticals and environmental toxins. PXR functions as a molecular sensor that activates the transcription of target genes by binding to various ligands, thereby increasing the expression of cytochrome P450 enzymes, transporters, and other proteins necessary for drug clearance. Understanding the structure and function of NR1I2 is critical for elucidating mechanisms of drug-drug interactions, variability in drug metabolism among individuals, and the development of therapeutics that can modulate this receptor for improved drug efficacy and safety. Recombinant NR1I2 proteins are essential for these studies, as they provide a platform for in vitro assays, structural biology investigations, and high-throughput screening of potential ligands. Advances in the production and characterization of NR1I2 recombinant proteins have the potential to facilitate drug discovery and personalized medicine by allowing researchers to better predict how different individuals might respond to medications based on their genetic makeup and the presence of various environmental factors. Consequently, ongoing research into NR1I2 not only enhances our understanding of pharmacology but also highlights the significance of nuclear receptors in human health and disease.
