Analytical Data
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Gene name
PRIM1
- Application
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Alternative Names
DNA primase 49KDA subunit Short name: p49
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49642
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Expression Region
1-420aa
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Molecular Weight
65.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRIM1, or Primase 1, is a pivotal enzyme involved in DNA replication, specifically in the synthesis of RNA primers essential for the initiation of DNA strand synthesis. Research on PRIM1 has intensified due to its critical role in maintaining genomic stability, which is vital for cellular proliferation and organismal development. Mutations or dysregulation of PRIM1 can lead to replication stress, contributing to various diseases, including cancer. Understanding the structure and function of PRIM1 and its interactions with other replication factors can provide insights into the mechanisms of DNA replication and repair. Additionally, PRIM1 serves as a potential target for therapeutic interventions, as manipulating its activity may enhance the effectiveness of chemotherapeutic agents. Notably, investigations into the recombinant expression of PRIM1 facilitate the production of large quantities of the protein, enabling detailed biochemical and biophysical studies. These studies aim to elucidate the enzyme's mechanism of action, its regulation, and its role in the broader context of DNA metabolism. Through these efforts, researchers hope to better understand the molecular underpinnings of diseases associated with DNA replication failures and to develop innovative strategies for treatment and prevention.
