Analytical Data
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Gene name
Frizzled-8
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简介
Frizzled-8 is a Wnt receptor that critically coordinates β-catenin signaling through the complex Wnt-Fzd-LRP5-LRP6 complex. It acts through the canonical Wnt/β-catenin pathway, activating scrambled proteins, inhibiting GSK-3 kinase, inducing nuclear β-catenin accumulation, and activating Wnt target genes. Frizzled-8 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-8 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Loaded Vantictumab (HY-P9974) on AHC2 biosensor, can bind Frizzled-8 Protein, Human (HEK293, His) with an affinity constant of 1.183E-07 M as determined in BLI assay. Loaded Vantictumab (HY-P9974) on AHC2 biosensor, can bind Frizzled-8 Protein, Human (HEK293, His) with an affinity constant of 1.183E-07 M as determined in BLI assay.
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Alternative Names
frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H461
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Expression Region
A28-P172
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Protein Length
Partial
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Molecular Weight
22-32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-8 (Fzd8) is a member of the Frizzled family of receptors, which play a crucial role in the Wnt signaling pathway, a key regulator of various developmental processes and cellular functions. The Wnt pathway is essential in governing cell fate determination, proliferation, and migration during embryonic development, as well as maintaining tissue homeostasis in adults. Fzd8, in particular, has been implicated in processes such as neural development, bone formation, and stem cell maintenance. Research into Fzd8 recombinant protein has gained momentum due to its potential applications in regenerative medicine and cancer therapy. Understanding the structural and functional aspects of Fzd8 can provide insights into its interactions with Wnt ligands and downstream signaling mechanisms. The production of Fzd8 recombinant protein allows for in vitro studies, facilitating the exploration of its role in various biological contexts and its therapeutic potential. As researchers continue to unravel the complexities of the Wnt signaling pathway, Fzd8 stands out as a significant target for developing novel strategies to manipulate cell signaling for improved treatment outcomes in diseases marked by aberrant Wnt activity, including cancer and congenital disorders.
