Analytical Data
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基因名
Frizzled-9
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简介
Frizzled-9 is a WNT2 receptor that is critical for activation of the β-catenin pathway, involved in Disheveled protein activation, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. In addition to classical signaling, it negatively regulates acetylcholine receptor clustering in neuromuscular junction assembly and affects the viability of neural progenitor cells by regulating the cell cycle. Frizzled-9 Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived Frizzled-9 protein, expressed by HEK293 , with C-mFc labeled tag.
- Application
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生物活性
Measured by its ability to bind biotinylated mouse Wnt-3a in a funtional ELISA with an estimated Kd 2.263 nM. Immobilized Frizzled-9 at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Wnt3a protein. The Kd is 2.263 nM.
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别名
Frizzled-9; Fzd9; Fz-9; mFz3; mFz9; CD349; Fzd3
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种属
Mouse
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表达系统
HEK293
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标签
C-mFc
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9R216
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表达区间
L24-D186
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蛋白长度
Partial
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分子量
52-61 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-9 (Fzd9) is a member of the Frizzled family of proteins, which serve as receptors for Wnt signaling pathways, playing crucial roles in various biological processes such as cell proliferation, differentiation, and tissue homeostasis. Abnormalities in Wnt signaling have been associated with several diseases, including cancer and developmental disorders, making Frizzled proteins significant targets for therapeutic intervention. Recent studies have highlighted the specific functions of Fzd9 in regulating neuronal development and synaptic plasticity, suggesting its potential impact on neurodegenerative diseases and psychiatric disorders. The recombinant expression of Frizzled-9 protein has gained attention in the research community, enabling scientists to investigate its structural characteristics and functional interactions with Wnt ligands and intracellular signaling molecules. Understanding the precise mechanisms by which Fzd9 modulates Wnt signaling could provide insights into the development of novel treatments for diseases linked to dysregulated Frizzled signaling pathways. This growing body of research underscores the importance of Fzd9 not only as a critical component of Wnt signaling but also as a valuable resource for elucidating complex biological interactions and advancing therapeutic strategies.
