Analytical Data
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Gene name
Frizzled-9
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简介
Frizzled-9 is a WNT2 receptor that is critical for activation of the β-catenin pathway, involved in Disheveled protein activation, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. In addition to classical signaling, it negatively regulates acetylcholine receptor clustering in neuromuscular junction assembly and affects the viability of neural progenitor cells by regulating the cell cycle. Frizzled-9 Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived Frizzled-9 protein, expressed by HEK293 , with C-mFc labeled tag.
- Application
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Biological Activity
Measured by its ability to bind biotinylated mouse Wnt-3a in a funtional ELISA with an estimated Kd 2.263 nM. Immobilized Frizzled-9 at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Wnt3a protein. The Kd is 2.263 nM.
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Alternative Names
Frizzled-9; Fzd9; Fz-9; mFz3; mFz9; CD349; Fzd3
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Species
Mouse
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Source
HEK293
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Tag
C-mFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9R216
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Expression Region
L24-D186
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Protein Length
Partial
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Molecular Weight
52-61 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-9 (Fzd9) is a member of the Frizzled family of proteins, which serve as receptors for Wnt signaling pathways, playing crucial roles in various biological processes such as cell proliferation, differentiation, and tissue homeostasis. Abnormalities in Wnt signaling have been associated with several diseases, including cancer and developmental disorders, making Frizzled proteins significant targets for therapeutic intervention. Recent studies have highlighted the specific functions of Fzd9 in regulating neuronal development and synaptic plasticity, suggesting its potential impact on neurodegenerative diseases and psychiatric disorders. The recombinant expression of Frizzled-9 protein has gained attention in the research community, enabling scientists to investigate its structural characteristics and functional interactions with Wnt ligands and intracellular signaling molecules. Understanding the precise mechanisms by which Fzd9 modulates Wnt signaling could provide insights into the development of novel treatments for diseases linked to dysregulated Frizzled signaling pathways. This growing body of research underscores the importance of Fzd9 not only as a critical component of Wnt signaling but also as a valuable resource for elucidating complex biological interactions and advancing therapeutic strategies.
