Analytical Data
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Gene name
ZIC4
- Application
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Alternative Names
ZIC4；Zinc finger Protein ZIC 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N9L1
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Expression Region
1-334aa
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AA Sequence
MRYKTSLVMRKRLRLYRNTLKESSSSSGHHGPQLTAASSPSVFPGLHEEPPQASPSRPLNGLLRLGLPGDMYARPEPFPPGPAARSDALAAAAALHGYGGMNLTVNLAAPHGPGAFFRYMRQPIKQELICKWLAADGTATPSLCSKTFSTMHELVTHVTVEHVGGPEQANHICFWEECPRQGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFRCEFEGCERRFANSSDRKKHSHVHTSDKPYTCKVRGCDKCYTHPSSLRKHMKVHGRSPPPSSGYDSATPSALVSPSSDCGHKSQVASSAAVAARTADLSE
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Molecular Weight
39.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ZIC4, a member of the ZIC (Zinc Finger of the Cerebellum) family of transcription factors, plays a critical role in early development, particularly in the formation of the central nervous system. Research into ZIC4 has gained significant attention due to its implications in various neurodevelopmental disorders and its potential as a biomarker for certain neurological conditions. Mutations in the ZIC4 gene have been linked to complex congenital conditions, including agenesis of the corpus callosum and other brain malformations. The protein's structure features multiple zinc-finger motifs, which facilitate its interaction with DNA and other proteins, suggesting that it acts as a transcriptional regulator during embryonic development. Studies aiming to understand the functional mechanisms of ZIC4 have utilized recombinant protein technologies to produce and characterize ZIC4 in vitro. These investigations are essential for elucidating ZIC4's regulatory pathways and identifying potential targets for therapeutic intervention. Understanding ZIC4's role not only advances the field of developmental biology but also opens avenues for research into treatments for related neurodevelopmental disorders.
