Analytical Data
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Gene name
KLHDC5
- Application
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Alternative Names
KLHL42; KIAA1340; KLHDC5Kelch-like protein 42; Cullin-3-binding protein 9; Ctb9; Kelch domain-containing protein 5
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9P2K6
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Expression Region
1-505aa
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AA Sequence
MSAEEMVQIRLEDRCYPVSKRKLIEQSDYFRALYRSGMREALSQEAGGPEVQQLRGLSAPGLRLVLDFINAGGAREGWLLGPRGEKGGGVDEDEEMDEVSLLSELVEAASFLQVTSLLQLLLSQVRLNNCLEMYRLAQVYGLPDLQEACLRFMVVHFHEVLCKPQFHLLGSPPQAPGDVSLKQRLREARMTGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEEMTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPSTNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSSDMWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFSDSWEAFRRFPAFGHNLLVSSLYLPNKAET
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Molecular Weight
55.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KLHDC5, or Kelch-like protein 5, is a member of the Kelch-like protein family, known for its role in various cellular processes, including cytoskeletal organization and signal transduction. The protein is characterized by its Kelch repeat domain, which is often involved in protein-protein interactions, suggesting its potential role as an adaptor in cellular pathways. Research into KLHDC5 has gained significance due to its implications in developmental biology and disease mechanisms, particularly in cancer and neurodegenerative disorders. Recent studies have reported its involvement in regulating key cellular functions such as cell proliferation, differentiation, and apoptosis, highlighting its potential as a therapeutic target. Moreover, understanding the structural and functional properties of KLHDC5 through recombinant protein studies can provide insights into its biological roles and how dysregulation may contribute to disease states. Investigating KLHDC5's interactions with other cellular proteins and its functional outcomes will be crucial in elucidating its contributions to health and disease, paving the way for potential innovative treatment strategies.











