Analytical Data
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Gene name
SEC31B
- Application
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Alternative Names
SEC31L2; Gm341; Protein transport protein Sec31B; SC31B_HUMAN; SEC31 B; SEC31 homolog B (S. cerevisiae); SEC31 homolog B; SEC31 like 2; SEC31 related protein B; SEC31-like protein 2; SEC31-related protein B; SEC31B 1; SEC31B; SEC31B-1; Secretory pathway component Sec31B 1; secretory pathway component Sec31B1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NQW1
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Expression Region
1-348 aa
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AA Sequence
MKLKELERPAVQAWSPASQYPLYLATGTSAQQLDSSFSTNGTLEIFEVDFRDPSLDLKHRGVLSALSRFHKLVWGSFGSGLLESSGVIAGGGDNGMLILYNVTHILSSGKEPVIAQKQKHTGAVRALDFNPFQGNLLASGASDSEIFIWDLNNLNVPMTLGSKSQPPEDIKALSWNRQAQHILSSAHPSGKAVVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDIATQLVLCSEDDRLPVIQLWDLRFASSPLKVLESHSRGILSVSWSQADAELLLTSAKDSQILCRNLGSSEVVYKLPTQSSWCFDVQWCPRDPSVFSAASFNGWISLYAVMGRSWEVQHMRQADKV
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Molecular Weight
64.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SEC31B, a member of the SEC31 protein family, plays a crucial role in the secretory pathway of eukaryotic cells, particularly in the formation of the coatomer complex during vesicle budding from the endoplasmic reticulum. This protein is essential for the transport of proteins and lipids from the endoplasmic reticulum to the Golgi apparatus, thereby facilitating cellular communication, secretion processes, and overall membrane dynamics. Research into SEC31B has garnered attention due to its potential links to various diseases, including certain neurodegenerative disorders and metabolic syndromes, where dysfunctional protein transport may contribute to cellular stress and pathology. Furthermore, SEC31B's involvement in the regulation of cellular homeostasis and its interaction with other proteins in the secretory pathway highlights its significance in maintaining proper cellular function. Investigating SEC31B at the molecular level could yield insights into its mechanisms and regulatory roles, ultimately leading to the development of therapeutic interventions aimed at rectifying transport-related disorders. Given its pivotal role, the recombinant expression of SEC31B protein has become an important avenue for research, enabling scientists to explore its structure-function relationship, interaction dynamics with other proteins, and implications in health and disease, thereby contributing to the broader understanding of cellular transport mechanisms.
