Analytical Data
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Gene name
B3GALT4
- Application
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Alternative Names
B3GALT4; GALT4; Beta-1.3-galactosyltransferase 4; Beta-1.3-GalTase 4; Beta3Gal-T4; Beta3GalT4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O96024
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Expression Region
1-378aa
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AA Sequence
MQLRLFRRLLLAALLLVIVWTLFGPSGLGEELLSLSLASLLPAPASPGPPLALPRLLIPNQEACSGPGAPPFLLILVCTAPENLNQRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQAAFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEPQREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGTGYVLSASAVQLILKVASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYGKFLLTSHRLDPWKMQEAWKLVGGSDGERTAPFCSWFQGVLGILRCRAIAWLQS
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Molecular Weight
67.32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
B3GALT4, a member of the β1,3-galactosyltransferase family, plays a crucial role in glycoprotein and glycolipid biosynthesis by catalyzing the transfer of galactose to various substrates. Its significance has been underscored by its involvement in congenital disorders of glycosylation, particularly as mutations in the B3GALT4 gene can lead to a range of clinical manifestations, including developmental delays and neurological issues. Research on the recombinant B3GALT4 protein has been pivotal in understanding its enzymatic mechanisms and substrate specificity. By producing this protein in a laboratory setting, researchers can investigate its structural properties and functional activities, which could reveal insights into its biological roles and potential therapeutic applications. Furthermore, understanding B3GALT4's interactions with other glycosyltransferases and its influence on glycan structures may help elucidate the complex pathways of glycosylation that are vital for cellular communication and function. As research progresses, the recombinant B3GALT4 protein serves as a valuable tool for both basic research and the development of diagnostic and treatment strategies for glycosylation-related diseases.
