Analytical Data
-
Gene name
CUL5
- Application
-
Alternative Names
CUL-5;Vasopressin-activated calcium-mobilizing receptor 1;VACM-1
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q93034
-
Expression Region
1-780aa
-
AA Sequence
MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA
-
Molecular Weight
97.9 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CUL5 (Cullin 5) is a member of the Cullin family of proteins that play a crucial role in the ubiquitin-proteasome system, which is responsible for the regulated degradation of proteins within the cell. As a key component of E3 ubiquitin ligase complexes, CUL5 facilitates the ubiquitination of target substrates, thereby influencing various cellular processes such as signal transduction, cell cycle regulation, and immune responses. Recent research has highlighted CUL5's involvement in various pathological conditions, including cancer, viral infections, and autoimmune diseases. Notably, CUL5 has been implicated in the regulation of viral proteins, particularly in the context of HIV and other retroviruses, where it aids in the degradation of host proteins that inhibit viral replication. The characterization of CUL5 and its interactions at the molecular level has opened avenues for therapeutic interventions targeting the ubiquitin-proteasome pathway. Understanding CUL5's structural properties and functional mechanisms is essential for developing novel strategies to manipulate its activity in disease contexts. Furthermore, CUL5's potential as a biomarker for certain diseases underscores the importance of ongoing research into its biological functions and regulatory mechanisms. As such, creating recombinant forms of CUL5 for study can significantly advance our understanding of its physiological roles and therapeutic potential in diverse diseases.
